Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span the diversity of the eukaryotic tree of life. However, genetic tractability has been limited to a few species, which do not represent eukaryotic diversity or environmentally relevant taxa. Here, we report on the development of genetic tools in a range of protists primarily from marine environments. We present evidence for foreign DNA delivery and expression in 13 species never before transformed and for advancement of tools for eight other species, as well as potential reasons for why transformation of yet another 17 species tested was not achieved. Our resource in genetic manipulation will provide insights into the ancestral eukaryotic lifeforms, general eukaryote cell biology, protein diversification and the evolution of cellular pathways.
Drahomíra Faktorová, R. Ellen R. Nisbet, José Fernández Robledo, Elena Casacuberta, Lisa Sudek, et al.. Genetic tool development in marine protists: emerging model organisms for experimental cell biology. Nature Methods, 2020, 17 (5), pp.481-494. ⟨10.1038/s41592-020-0796-x⟩ (lien externe). ⟨hal-03018835⟩ (lien externe)
Citations
APA
Faktorová, D., Nisbet, R. E. R., Fernández Robledo, J., Casacuberta, E., Sudek, L., Allen, A., Ares, M., Aresté, C., Balestreri, C., Barbrook, A., Beardslee, P., Bender, S., Booth, D., Bouget, F.-Y., Bowler, C., Breglia, S., Brownlee, C., Burger, G., Cerutti, H., … Lukeš, J. (2020). Genetic tool development in marine protists: emerging model organisms for experimental cell biology. In Nature Methods. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7200600
MLA
Faktorová, Drahomíra, et al. “Genetic Tool Development in Marine Protists: Emerging Model Organisms for Experimental Cell Biology.” Nature Methods, May 2020, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7200600.
Chicago
Faktorová, Drahomíra, R. Ellen R. Nisbet, José Fernández Robledo, et al. 2020. “Genetic Tool Development in Marine Protists: Emerging Model Organisms for Experimental Cell Biology.” In Nature Methods. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7200600.
Harvard
Faktorová, D. et al. (2020) “Genetic tool development in marine protists: emerging model organisms for experimental cell biology,” Nature Methods. Available at: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7200600.
ISO 690
FAKTOROVÁ, Drahomíra, NISBET, R. Ellen R., FERNÁNDEZ ROBLEDO, José, CASACUBERTA, Elena, SUDEK, Lisa, ALLEN, Andrew, ARES, Manuel, ARESTÉ, Cristina, BALESTRERI, Cecilia, BARBROOK, Adrian, BEARDSLEE, Patrick, BENDER, Sara, BOOTH, David, BOUGET, François-Yves, BOWLER, Chris, BREGLIA, Susana, BROWNLEE, Colin, BURGER, Gertraud, CERUTTI, Heriberto, CESARONI, Rachele, CHIURILLO, Miguel, CLEMENTE, Thomas, COLES, Duncan, COLLIER, Jackie, COONEY, Elizabeth, COYNE, Kathryn, DOCAMPO, Roberto, DUPONT, Christopher, EDGCOMB, Virginia, EINARSSON, Elin, ELUSTONDO, Pía, FEDERICI, Fernan, FREIRE-BENEITEZ, Veronica, FREYRIA, Nastasia J., FUKUDA, Kodai, GARCÍA, Paulo, GIRGUIS, Peter, GOMAA, Fatma, GORNIK, Sebastian, GUO, Jian, HAMPL, Vladimír, HANAWA, Yutaka, HARO-CONTRERAS, Esteban, HEHENBERGER, Elisabeth, HIGHFIELD, Andrea, HIRAKAWA, Yoshihisa, HOPES, Amanda, HOWE, Christopher, HU, Ian, IBAÑEZ, Jorge, IRWIN, Nicholas, ISHII, Yuu, JANOWICZ, Natalia Ewa, JONES, Adam, KACHALE, Ambar, FUJIMURA-KAMADA, Konomi, KAUR, Binnypreet, KAYE, Jonathan, KAZANA, Eleanna, KEELING, Patrick, KING, Nicole, KLOBUTCHER, Lawrence, LANDER, Noelia, LASSADI, Imen, LI, Zhuhong, LIN, Senjie, LOZANO, Jean-Claude, LUAN, Fulei, MARUYAMA, Shinichiro, MATUTE, Tamara, MICELI, Cristina, MINAGAWA, Jun, MOOSBURNER, Mark, NAJLE, Sebastián, NANJAPPA, Deepak, NIMMO, Isabel, NOBLE, Luke, NOVÁK VANCLOVÁ, Anna, NOWACKI, Mariusz, NUÑEZ, Isaac, PAIN, Arnab, PIERSANTI, Angela, PUCCIARELLI, Sandra, PYRIH, Jan, REST, Joshua, RIUS, Mariana, ROBERTSON, Deborah, RUAUD, Albane, RUIZ-TRILLO, Iñaki, SIGG, Monika, SILVER, Pamela, SLAMOVITS, Claudio, JASON SMITH, G., SPRECHER, Brittany, STERN, Rowena, SWART, Estienne, TSAOUSIS, Anastasios, TSYPIN, Lev, TURKEWITZ, Aaron, TURNŠEK, Jernej, VALACH, Matus, VERGÉ, Valérie, VON DASSOW, Peter, VON DER HAAR, Tobias, WALLER, Ross, WANG, Lu, WEN, Xiaoxue, WHEELER, Glen, WOODS, April, ZHANG, Huan, MOCK, Thomas, WORDEN, Alexandra and LUKEŠ, Julius, 2020. Genetic tool development in marine protists: emerging model organisms for experimental cell biology [en ligne]. May 2020. Disponible à l'adresse : https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7200600